Aim: To partial purify and determine antifungal activity of Neisseria chitinases. Materials and Methods: Characterized and optimized chitinase were obtained from Neisseria species and partially purified by ammonium sulphate precipitation, dialysis and SDS-PAGE for molecular weight determination. Further antifungal activities were determined against Trichoderma sp, Aspergillus sp and candida sp by agar well diffusion method at 25°c. Results: The partially purified chitinase showed 43kDa molecular weight. Half-life of the chitinase enzyme was three and half hours at 28°c and showed effective antifungal activity against Candida sp, Aspergillus sp and Tricoderma sp.