Objective of this study to assess the influence of seed priming on biochemical traits of maize (Zea mays L.). The experiment was carried out during kharif 2016 at research Farm JNKVV, Jabalpur (MP) which was laid out in a randomized block design replicated thrice. The treatments comprised of nine seed priming treatments in maize CV, African tall viz., water, ZnSO₄ @ 0.5%, KNO₃ @ 0.5% &KH₂PO₄ @ 0.5% for 6 and 12 hrs and control which were assessed for biochemical studies. The seed priming treatments showed variable response for most of the traits. Water primed for 12 hrs indicated the highest values for protein (10.51%), fat (3.34%) and ash (1.77%). KNO₃ for 12 hours showed high values for crude fiber (6.07%) and carbohydrates (73.46%).